Measurements of noise in Josephson-effect mixers

We present new heterodyne receiver results obtained at 100 GHz using resistively-shunted Nb and NbN tunnel junctions. In addition, we have carried out accurate measurements of the available noise power of these devices at the L-band (1.5 GHz) IF frequency. Both the heterodyne and the output noise measurements show that the noise of these devices can be a factor of five or more higher than that predicted by the simple current-biased RSJ model. The noise approaches the appropriate thermal or thermal and shot noise limits for bias voltages where the nonlinearity is not strong (i.e., V>ICRN), but as expected from the RSJ model, can be significantly higher at the low voltages where the mixers are typically biased. The bias voltage dependence of the noise shows structure which is associated with resonances in the RF embedding circuit. Surprisingly, we find that changes in the high-frequency (100 GHz) impedance presented to the junction can dramatically affect the magnitude and voltage dependence of the low-frequency (1.5 GHz) noise. This emphasizes the necessity of very closely matching the junction to free space over a wide frequency range

The Alignment of the Magnetic Field and Collimated Outflows in Star-forming Regions: the Case of NGC 2071

The magnetic field is believed to play a crucial role in the process of star formation. From the support it provides during the initial collapse of molecular clouds to the creation of strong collimated jets responsible for large mass losses, current theories predict its importance in many different stages during the formation of stars. Here we report on observational evidence which tests one aspect that can be inferred from these theories: the alignment between the local magnetic field and collimated bipolar outflows in such environments. There is good evidence of an alignment in the case of NGC 2071.Comment: 11 pages, 4 figure

ACTIVE SUPPRESSION AS A POSSIBLE MECHANISM OF TOLERANCE IN TETRAPARENTAL MICE

Previous work has indicated that tetraparental mice, chimeric since the eight-cell stage because of embryo fusion using histoincompatible strain combinations, possess autospecific immune cells and blocking antibodies. Although this phenomenon has been demonstrated in vitro, it may have relevance to the self-tolerance shown by these mice in vivo. The experiments described here indicate that spleen cells from tetraparental mice can block mixed lymphocyte reactions between the two parental cell types, but not between unrelated strains. Furthermore, this suppressive ability is not affected by an otherwise effective treatment of the tetraparental spleen cells with anti-θ antibody and complement. The in vitro experimental system elaborated here should help to characterize the cell type responsible for the suppression

Autonomous Detection of the Loss of a Wing for Underwater Gliders

Over the past five years, two of the Slocum underwater gliders operated by the UK National Oceanography Centre have lost a wing mid-mission without the pilot being aware of the problem until the point of vehicle retrieval. In this study, the steady-state data collected by gliders during the two deployments has been analysed to develop a fault detection system. From the data analysis, it is clear that the loss of the wing was a sudden event for both gliders. The main changes to the system dynamics associated with the event are an increase in the positive buoyancy of the glider and the occurrence of a roll angle on the side of the lost wing, with significant difference between dives and climbs. Hence, a simple effective system for the detection of the wing loss has been designed using the roll angle. Since sensors are known to fail and the roll sensor is non-critical to the operation of the glider, a back-up diagnostics system has been developed based on the dynamic model of the vehicle, capturing the change in buoyancy. Both systems are able to correctly detect the loss of the wing and notify pilots, who can re-plan missions to safely recover the vehicle

Diagnostic Molecular Markers for Phosphine Resistance in US Populations of Tribolium castaneum and Rhyzopertha dominica

Citation: Chen, Z., Schlipalius, D., Opit, G., Subramanyam, B., & Phillips, T. W. (2015). Diagnostic Molecular Markers for Phosphine Resistance in US Populations of Tribolium castaneum and Rhyzopertha dominica. Plos One, 10(3), 14. doi:10.1371/journal.pone.0121343Stored product beetles that are resistant to the fumigant pesticide phosphine (hydrogen phosphide) gas have been reported for more than 40 years in many places worldwide. Traditionally, determination of phosphine resistance in stored product beetles is based on a discriminating dose bioassay that can take up to two weeks to evaluate. We developed a diagnostic cleaved amplified polymorphic sequence method, CAPS, to detect individuals with alleles for strong resistance to phosphine in populations of the red flour beetle, Tribolium castaneum, and the lesser grain borer, Rhyzopertha dominica, according to a single nucleotide mutation in the dihydrolipoamide dehydrogenase (DLD) gene. We initially isolated and sequenced the DLD genes from susceptible and strongly resistant populations of both species. The corresponding amino acid sequences were then deduced. A single amino acid mutation in DLD in populations of T. castaneum and R. dominica with strong resistance was identified as P45S in T. castaneum and P49S in R. dominica, both collected from northern Oklahoma, USA. PCR products containing these mutations were digested by the restriction enzymes MboI and BstNI, which revealed presence or absence, respectively of the resistant (R) allele and allowed inference of genotypes with that allele. Seven populations of T. castaneum from Kansas were subjected to discriminating dose bioassays for the weak and strong resistance phenotypes. Application of CAPS to these seven populations confirmed the R allele was in high frequency in the strongly resistant populations, and was absent or at a lower frequency in populations with weak resistance, which suggests that these populations with a low frequency of the R allele have the potential for selection of the strong resistance phenotype. CAPS markers for strong phosphine resistance will help to detect and confirm resistant beetles and can facilitate resistance management actions against a given pest population

Transcription by the numbers redux: experiments and calculations that surprise

The study of transcription has witnessed an explosion of quantitative effort both experimentally and theoretically. In this article we highlight some of the exciting recent experimental efforts in the study of transcription with an eye to the demands that such experiments put on theoretical models of transcription. From a modeling perspective, we focus on two broad classes of models: the so-called thermodynamic models that use statistical mechanics to reckon the level of gene expression as probabilities of promoter occupancy, and rate-equation treatments that focus on the temporal evolution of the activity of a given promoter and that make it possible to compute the distributions of messenger RNA and proteins. We consider several appealing case studies to illustrate how quantitative models have been used to dissect transcriptional regulation

Probing the Magnetic Field with Molecular Ion Spectra

Observations of the effect of the magnetic field on its environment are usually achieved with techniques which rely on the interaction with the spin of the particles under study. Because of the relative weakness of this effect, extraction of the field characteristics proves to be a most challenging task. We take a totally different approach to the problem and show that the manifestation of the magnetic field can be directly observed by means of a comparison of the spectra of molecular ions with those of neutral molecules. This takes advantage of the strong cyclotron interaction between the ions and the field, but requires the presence of flows or turbulent motion in the gas. We compare our theory to data obtained on the OMC-1, OMC-2, OMC-3 and DR21(OH) molecular clouds.Comment: 16 pages, 3 figures, 2 table

A remote anomaly detection system for Slocum underwater gliders

Marine Autonomous Systems (MAS) operating at sea beyond visual line of sight need to be self-reliant, as any malfunction could lead to loss or pose a risk to other sea users. In the absence of fully automated on-board control and fault detection tools, MAS are piloted and monitored by experts, resulting in high operational costs and limiting the scale of observational fleets that can be deployed simultaneously. Hence, an effective anomaly detection system is fundamental to increase fleet capacity and reliability. In this study, an on-line, remote fault detection system is developed for underwater gliders. Two alternative methods are analysed using time series data: feedforward deep neural networks estimating the glider’s vertical velocity and an autoencoder. The systems are trained using field data from four baseline deployments of Slocum gliders and tested on six deployments of vehicles suffering from adverse behaviour. The methods are able to successfully detect a range of anomalies in the near real time data streams, whilst being able to generalise to different glider configurations. The autoencoder’s error in reconstructing the original signals is the clearest indicator of anomalies. Thus, the autoencoder is a prime candidate to be included into an all-encompassing condition monitoring system for MAS

The triton and three-nucleon force in nuclear lattice simulations

We study the triton and three-nucleon force at lowest chiral order in pionless effective field theory both in the Hamiltonian and Euclidean nuclear lattice formalism. In the case of the Euclidean lattice formalism, we derive the exact few-body worldline amplitudes corresponding to the standard many-body lattice action. This will be useful for setting low-energy coefficients in future nuclear lattice simulations. We work in the Wigner SU(4)-symmetric limit where the S-wave scattering lengths {1}S{0} and {3}S{1} are equal. By comparing with continuum results, we demonstrate for the first time that the nuclear lattice formalism can be used to study few-body nucleon systems.Comment: 16 pages, 4 figure